Vibrio cholerae

Vibrio cholerae can cause syndromes ranging from asymptomatic to cholera gravis. Symptoms include abrupt onset of watery diarrhoea, occasional vomiting and abdominal cramps. Dehydration ensues with symptoms and signs such as thirst, dry mucous membranes, decreased skin turgor, sunken eyes, hypotension, weak pulse, tachycardia, tachypnea, hoarse voice, oliguria, cramps, renal failure, seizures, somnolence, coma and death.

This PCR kit enables to detect V. cholerae DNA (if Hly sequence is present) and to identify pathogenic V. cholerae strains (if main virulence factors - CtxA, tcpA are present), belonging to serogroups O1 (if amplification target wbeT is present), or O139 (if amplification target wbfR is present). Each PCR kit contains two detection forms - Screen kit form - enables amplification of CtxA target (FAM/Green), tcpA target (ROX/Orange) and IC target (JOE/Yellow/HEX), form Type kit form enables amplification of Hly target (JOE/Yellow/HEX) - cholera germs of all groups, wbeT (FAM/ Green) - belonging to serogroup O1 and wbf (ROX/Orange) - belonging to serogroup O139. It is necessary to carry out both “Screen” and “Type” reactions for valid results interpretation.


New CE
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Type Pack Cat. No. Name Description Kit
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 CE Real Time PCR Only R-B53(RG)-CEVibrio cholerae-FRT
PDF
Vibrio cholerae DNA Real Time amplification kit
Aliquoted in tubes55
Real TimeFEP KitsElectrophoresis KitsAla 1/4 Detector