Hepatitis virus B

Hepatitis B virus (HBV) is a species of the genus Orthohepadnavirus, which is likewise a part of the Hepadnaviridae family of viruses. The virus is divided into four major serotypes (adr, adw, ayr, ayw) based on antigenic epitopes present on its envelope proteins and into eight genotypes (labeled A through H) according to overall nucleotide sequence variation of the genome. The genotypes have a distinct geographical distribution and are used in tracing the evolution and transmission of the virus. Differences between genotypes affect the disease severity, course and likelihood of complications and response to treatment and possibly vaccination. A possible new "I" genotype has been described, but acceptance of this notation is not universal. Different genotypes may respond to treatment in different ways. The life cycle of hepatitis B virus is complex. HBV is one of a few known non-retroviral viruses which use reverse transcription as a part of its replication process.

Hepatitis B is an infectious illness caused by HBV which infects the liver of hominoidea, including humans and causes an inflammation called hepatitis. Originally was known as "serum hepatitis". Transmission of HBV results from exposure to infectious blood or body fluids such as semen and vaginal fluids, while viral DNA has been detected in the saliva, tears and urine of chronic carriers with high titer DNA in serum. Perinatal infection is a major route of infection in endemic (mainly developing) countries. Other risk factors for developing HBV infection include working in a health care setting, transfusions and dialysis.

Acute infection with HBV is associated with acute viral hepatitis – an illness that begins with general ill-health, loss of appetite, nausea, vomiting, body aches, mild fever, dark urine and then progresses to development of jaundice. It has been noted that itchy skin has been an indication as a possible symptom of all hepatitis virus types. The illness lasts for a few weeks and then gradually improves in most affected people. A few patients may have more severe liver disease (fulminant hepatic failure) and may die as a result. The infection may be entirely asymptomatic and may go unrecognized.

Chronic infection with HBV may be either asymptomatic or may be associated with a chronic inflammation of the liver (chronic hepatitis), leading to cirrhosis over a period of several years. This type of infection dramatically increases the incidence of hepatocellular carcinoma (liver cancer). Hepatitis B virus has been linked to the development of Membranous glomerulonephritis (MGN).

HBV-FRT PCR kit (R-V5-Mod) is an in vitro nucleic acid amplification test for qualitative detection of HBV DNA in the clinical materials (blood plasma). The Internal Control is present in order to check all steps, including DNA extraction. IC amplification product is detected on the FAM/Green channel and HBV amplification product is detected in the JOE/Yellow/HEX channel. The analytical sensitivity depends on the DNA extraction system as well as on the initial sample volume (50 IU/ml if sample volume is 100 μl, 5 IU/ml if sample volume is 1000 μl).

HBV-Monitor-FRT PCR kit (R-V5-MC) is an in vitro nucleic acid amplification test for quantitative detection of HBV DNA in the clinical material (blood plasma).

The linear measurement range of HBV-Monitor-FRT PCR Kit is 15 - 100,000,000 IU/ml if sample volume is 1000 μl, or 150 - 100,000,000 IU/ml if sample volume is 100 μl.

New CE
Type Pack Cat. No. Name Description Kit
No. of
   Real Time PCR Only R-V5-Mod(RG,iQ,Mx,Dt)-CEHBV-FRT
HBV DNA Real Time amplification kitNon-aliquoted112
   Real Time PCR Only R-V5-G-F-CEHBV-genotype-FRT
HBV Genotypes A, B, C, D DNA Real Time PCR amplification and differentiation kit. 4 optical chanels are requiredNon-aliquoted55
   Real Time PCR Only R-V5-МC(RG,iQ,Mx,Dt)-CEHBV-Monitor-FRT
HBV DNA Real Time amplification kit. QUANTITATIVE. Includes Calibrator for internal control coefficient calculationNon-aliquoted80 (48 clinical samples)
Real TimeFEP KitsElectrophoresis KitsAla 1/4 Detector