Cryptococcus neoformans

Pneumocystis pneumonia (PCP) or pneumocystosis is a form of pneumonia, caused by the yeast-like fungus (which had previously been classified as a protozoan) Pneumocystis jirovecii. This pathogen is specific to humans; it has not been shown to infect other animals, while other species of Pneumocystis that parasitize other animals have not been shown to infect humans.

Pneumocystis is commonly found in the lungs of healthy people. The PCP disease is relatively rare in people with normal immune systems, but being a source of opportunistic infection it can cause a lung infection in people with a weak immune system, such as premature or severely malnourished children, the elderly and especially persons lwith HIV/AIDS, in whom it is most commonly observed. PCP can also develop in patients who are taking immunosuppressive medications. It can occur in patients who have undergone solid organ transplantation or bone marrow transplantation and after a surgery. Infections with Pneumocystis are also common in infants with hyper IgM syndrome, an X-linked or autosomal recessive trait.

Symptoms of PCP include fever, non-productive cough (because sputum is too viscous to become productive), shortness of breath (especially on exertion), weight loss and night sweats. There is usually not a large amount of sputum with PCP unless the patient has an additional bacterial infection. The fungus can invade other visceral organs, such as the liver, spleen and kidney, but only in a minority of cases. Pneumothorax is a well-known complication of PCP. An acute history of chest pain with breathlessness and diminished breath sounds is typical of pneumothorax.

Pneumocystis jirovecii (carinii) PCR kit is an in vitro nucleic acid amplification test for qualitative detection of Pneumocystis jirovecii (carinii) in the clinical material (bronchoalveolar lavage, sputum, biopsy material, throat washes and swabs) by Real-Time technology.

For detection, 2 channels – FAM/Green (for Internal Control) and JOE/Yellow/HEX (for P. jirovecii) are needed.

Analytical sensitivity of PCR kit is no less than 500 copies per 1 ml of sample.


New CE
mark
Type Pack Cat. No. Name Description Kit
format
No. of
tests
NewCE Real Time PCR Only R-F4-F(RG,iQ)-CECryptococcus neoformans-FRT
PDF
Cryptococcus neoformans DNA Real Time amplification kitNon-aliquoted110
Real TimeFEP KitsElectrophoresis KitsAla 1/4 Detector