HPV Infections

Human papillomaviruses (HPVs) are a group of more than 150 related viruses. They are called papillomaviruses because certain types may cause warts, or papillomas, which are benign (noncancerous) tumors.

Some HPVs, such as those that cause the common warts that grow on hands and feet, do not spread easily. However, more than 40 HPV types are sexually transmitted and these HPVs spread very easily through genital contact. Some types of sexually transmitted HPVs cause cervical cancer and other types of cancer. These are called high-risk, oncogenic, or carcinogenic HPVs. Other sexually transmitted types of HPV do not appear to cause cancer and are called low-risk HPVs.

Although genital HPV infections are very common, most occur without any symptoms and go away without any treatment within a few years. However, some HPV infections can persist for many years. Persistent infections with high-risk HPV types can cause cell abnormalities. If untreated, areas of abnormal cells (lesions) can in some cases develop into cancer.

Some types of sexually transmitted low-risk HPVs cause warts to appear on or around the genitals or anus. Most genital warts (condylomata acuminata) are caused by two HPV types, HPV-6 and HPV-11. Warts may appear within several weeks after sexual contact with a person who is infected with HPV, or they may take months or years to appear, or they may never appear.

AmpliSens® HPV HCR screen-titre-FRT (R-V31) PCR kit is capable to detect and quantify (without exact genotype detecting) the HPV DNA of two main phylogenetic groups – A7, A9, which include the following 10 types: 16, 18, 31, 33, 35, 39, 45, 52, 58, 59 – as well as the HPV DNA 51 (A5 group) and 56 (A6 group) types.

The method is based on simultaneous Real-time multiplex PCR and detection of E1-E2 HPV genes DNA fragments and a fragment of β-globin gene DNA which is used as internal endogenous control. For type 2x kit detection - JOE/Yellow and FAM/Green channels are needed and for type 4x kit - FAM/Green, JOE/Yellow, ROX/Orange and Cy5/Red channels are needed.

Analytical sensitivity is no less then 5 x 103 copies/ml.

AmpliSens® HPV HCR Genotype FRT (R-V25) - the detection and differentiation of 12 HPV HCR types - 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 is carried out in four tubes. Each HPV type is registered on its own channel that allows not only to detect, but also to differentiate the virus genotype. DNA target selected as Internal Control is a fragment of human β-globin and must always be present (as in all other HPV tests) in sample (cervical swab) in sufficient amount that is equal to the amount of cells in the smear (103 – 105 of genomes). For detection, FAM/Green, JOE/Yellow, ROX/Orange and Cy5/Red channels are needed.

Analytical sensitivity is no less then 1 x 103 copies/ml.

AmpliSens® HPV HCR Genotype titre FRT (R-V67) - the detection, differentiation and quantitation of 14 HPV HCR types - 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 is carried out in four tubes. Each HPV type is registered on its own channel that allows not only to detect, but also to differentiate the virus genotype and quantify genotype groups.

Analytical sensitivity is 1 x 103 copies/ml.

Therefore, endogenous Internal Control, present in all our HPV kits, allows not only control stages of PCR (DNA isolation and amplification) but also evaluate sample quality and storage adequacy. If epithelial swab quality is not sufficient (number of epithelial cells in the clinical sample is insufficient), signal of β-globin gene will be significantly lowered. Such β-globin based Internal Control significantly reduces false negative results, caused by a poor clinical sample quality.


New CE
mark
Type Pack Cat. No. Name Description Kit
format
No. of
tests
NewCE Real Time PCR Only R-V67-F-CEHPV HCR genotype-titre-FRT
PDF
HR HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 types Real Time PCR kit. (14 HPV genotypes). Differentiation and QUANTITATIVE detection of each genotype groupNon-aliquoted110
 CE Real Time PCR Only R-V31-T-2x(RG,iQ,SC)-CEHPV HCR screen-titre-FRT
PDF
HR HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 types DNA Real Time amplification kit. (12 HPV genotypes). QUANTITATIVE. 2 optical channels are requiredNon-aliquoted108
 CE Real Time PCR Only R-V31-T-4x(RG,iQ,Mx)-CEHPV HCR screen-titre-FRT
PDF
HR HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 types DNA Real Time amplification kit. QUANTITATIVE. 4 optical channels are requiredNon-aliquoted108
NewCE Real Time PCR Only R-V31-F-CEHPV HCR screen-titre-FRT
PDF
HR HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68 types Real Time amplification kit. (14 HPV genotypes). QUANTITATIVE. 5 optical channels are requiredNon-aliquoted110
 CE Real Time PCR Only R-V25(RG,iQ,Mx)-CEHPV HCR genotype-FRT
PDF
HR HPV 16, 18, 31 ,33, 35, 39, 45 ,51, 52, 56, 58, 59 types DNA Real Time amplification kit. Differentiation of 12 HPV genotypes. 4 optical channels are requiredNon-aliquoted108
 CE Real Time PCR Only R-V12(RG,iQ,Mx)-CEHPV 16/18-FRT
PDF
HPV DNA 16 and 18 genotypes DNA Real Time amplification kit. QUANTITATIVE. 3 optical channels are requiredNon-aliquoted108
 CE FEP PCR Only V31-FEP-CEHPV HCR screen-FEP
PDF
HR HPV 16, 18, 31, 33, 35, 39, 45, 52, 58, 59, 67 types DNA amplification kit. Adapted for АLА-1/4Non-aliquoted120
 CE FEP PCR Only V31-3x-FEP-CEHPV HCR screen-FEP
PDF
HR HPV 16, 18, 31, 33, 35, 39, 45, 52, 58, 59, 67 types DNA amplification kit. Adapted for АLА-1/4
Non-aliquoted120
 CE Elfo PCR Only V31-100F-CEHPV HCR screen-EPh
PDF
HR HPV 16, 18, 31, 33, 35, 39, 45, 52, 53, 56, 58, 59, 66, 70 types DNA amplification kitNon-aliquoted110
 CE Elfo PCR Only V25-50F-CEHPV HCR genotype-EPh
PDF
HR HPV 16, 18, 31, 33, 35, 39, 45, 52, 56, 58, 59, 66 types DNA amplification and Genotyping kit 0Non-aliquoted55
 CE Real Time PCR Only R-V11(RG,iQ,Mx)-CEHPV 6/11-FRT
PDF
HPV DNA 6 and 11 genotypes DNA Real Time amplification kit
Non-aliquoted120
 CE Elfo PCR Only V11-100-R0,2-CEHPV 6/11-EPh
PDF
HPV DNA 6 and 11 genotypes DNA amplification kit
Aliquoted in tubes110
Real TimeFEP KitsElectrophoresis KitsAla 1/4 Detector